Isg15 Polyclonal Antibody for Mouse(Mus musculus) | EIAab.comIsg15 Polyclonal Antibody for Mouse(Mus musculus) | EIAab.com

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Isg15 (Gene name),

Ubiquitin-like protein ISG15 (Protein name ), ISG15_MOUSE from NCBI database.

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General Annotation

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Antigen Annotation

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3D

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Predicted Eptitope

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Vaild Sequence

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Gene name:

Isg15(G1p2;Ucrp);

Protein name:

Ubiquitin-like protein ISG15;

Alternative:

Interferon-induced 17 kDa protein(IP17);Interferon-induced 15 kDa protein;Ubiquitin cross-reactive protein;

Organism:

Mouse (Mus musculus).

General Annotation

Sub Unit:

Interacts with, and is conjugated to its targets by the UBE1L (E1 enzyme) and UBE2E2 (E2 enzyme).

Function:

In response to IFN-tau secreted by the conceptus, may ligate to and regulate proteins involved in the release of prostaglandin F2-alpha (PGF), and thus prevent lysis of the corpus luteum and maintain the pregnancy.

Subcellular Location:

Cytoplasm Secreted UCRP conjugates seem to be noncovalently associated with the intermediate filaments and distributed in a punctate pattern. Also secreted.

Protein Attributes:

Sequence length:

161

Sequence:

50:

MAWDLKVKML | GGNDFLVSVT | NSMTVSELKK | QIAQKIGVPA | FQQRLAHQTA |

100:

VLQDGLTLSS | LGLGPSSTVM | LVVQNCSEPL | SILVRNERGH | SNIYEVFLTQ |

150:

TVDTLKKKVS | QREQVHEDQF | WLSFEGRPME | DKELLGEYGL | KPQCTVIKHL |

161:

RLRGGGGDQC | A

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

String:

UniGene:

SMR:

KEGG:

Pfam:

Uniprot:

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ELISA Kit for Mouse Ubiquitin-like protein ISG15

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E14926h

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96T

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ELISA Kit for Mouse Ubiquitin-like protein ISG15

Cat.:

E14926b

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ELISA Kit for Mouse Ubiquitin-like protein ISG15

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E14926m

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CLIA Kit for Mouse Ubiquitin-like protein ISG15

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U14926m

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96T

CLIA Kit for Mouse Ubiquitin-like protein ISG15

Cat.:

U14926h

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CLIA Kit for Mouse Ubiquitin-like protein ISG15

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U14926b

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Polyclonal Antibody for Mouse Ubiquitin-like protein ISG15

Polyclonal Antibody for Mouse Ubiquitin-like protein ISG15

Polyclonal Antibody for Mouse Ubiquitin-like protein ISG15

Monoclonal Antibody for Mouse Ubiquitin-like protein ISG15

Monoclonal Antibody for Mouse Ubiquitin-like protein ISG15

Monoclonal Antibody for Mouse Ubiquitin-like protein ISG15

Protein for Mouse Ubiquitin-like protein ISG15

Protein for Mouse Ubiquitin-like protein ISG15

Protein for Mouse Ubiquitin-like protein ISG15

R&D Technical Data

s

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s

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.3%

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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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Recovery

Recovery was determined by spiking various levels of ISG15 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	104	96-107
Plasma	103	94-106

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Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ISG15 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	99-108%	98-111%	100-106%	99-109%
EDTA plasma(n=5)	95-111%	97-108%	98-108%	100-107%
heparin plasma(n=5)	98-106%	94-112%	96-109%	98-108%

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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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References

1.

"Nucleotide sequence and deduced amino acid sequence of a cDNA encoding an interferon-induced mouse 15-KDa protein."
Fahey D.
Submitted (1992-10) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
strain: BALB/c.
2.

"Sequence differences between murine ISG15, human ISG15 and ubiquitin: a functional divergence from ubiquitin."
Garlie N.K. , Haas A.L. , D'Cunha J. , Schilling D. , Knight E. Jr. , Borden E.C.
Submitted (1996-06) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
3.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
tissue: Mammary gland.
4.

"Serpin 2a is induced in activated macrophages and conjugates to a ubiquitin homolog."
Hamerman J.A. , Hayashi F. , Schroeder L.A. , Gygi S.P. , Haas A.L. , Hampson L. , Coughlin P. , Aebersold R. , Aderem A.
J. Immunol.168:2415-2423(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 9-30; 36-44 AND 89-106;CONJUGATION TO SERPINA3G
5.

"Identification of a ubiquitin family protein as a novel neutrophil chemotactic factor."
Owhashi M. , Taoka Y. , Ishii K. , Nakazawa S. , Uemura H. , Kambara H.
Biochem. Biophys. Res. Commun.309:533-539(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PARTIAL PROTEIN SEQUENCE;CHEMOTACTIC ACTIVITY;MASS SPECTROMETRY
6.

"High-throughput immunoblotting. Ubiquitin-like protein ISG15 modifies key regulators of signal transduction."
Malakhov M.P. , Kim K.I. , Malakhova O.A. , Jacobs B.S. , Borden E.C. , Zhang D.-E.
J. Biol. Chem.278:16608-16613(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: CONJUGATION TO JAK1; MAPK3 AND PLCG1
7.

"Identification of interferon-stimulated gene 15 as an antiviral molecule during Sindbis virus infection in vivo."
Lenschow D.J. , Giannakopoulos N.V. , Gunn L.J. , Johnston C. , O'Guin A.K. , Schmidt R.E. , Levine B. , Virgin H.W. IV
J. Virol.79:13974-13983(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN SINDBIS VIRUS RESTRICTION
8.

"Negative regulation of ISG15 E3 ligase EFP through its autoISGylation."
Zou W. , Wang J. , Zhang D.-E.
Biochem. Biophys. Res. Commun.354:321-327(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN TRIM25 ISGYLATION
9.

"ISG15 modification of the eIF4E cognate 4EHP enhances cap structure-binding activity of 4EHP."
Okumura F. , Zou W. , Zhang D.E.
Genes Dev.21:255-260(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN EIF4E2 ISGYLATION
10.

"IFN-stimulated gene 15 functions as a critical antiviral molecule against influenza, herpes, and Sindbis viruses."
Lenschow D.J. , Lai C. , Frias-Staheli N. , Giannakopoulos N.V. , Lutz A. , Wolff T. , Osiak A. , Levine B. , Schmidt R.E. , Garcia-Sastre A. , Leib D.A. , Pekosz A. , Knobeloch K.P. , Horak I. , Virgin H.W. IV
Proc. Natl. Acad. Sci. U.S.A.104:1371-1376(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN HHV-1; SV; INFLUENZA A AND B VIRUS RESTRICTION
11.

"Nitrosylation of ISG15 prevents the disulfide bond-mediated dimerization of ISG15 and contributes to effective ISGylation."
Okumura F. , Lenschow D.J. , Zhang D.E.
J. Biol. Chem.283:24484-24488(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBUNIT;S-NITROSYLATION AT CYS-76 AND CYS-144
12.

"Negative feedback regulation of RIG-I-mediated antiviral signaling by interferon-induced ISG15 conjugation."
Kim M.J. , Hwang S.Y. , Imaizumi T. , Yoo J.Y.
J. Virol.82:1474-1483(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN DDX58 ISGYLATION
13.

"Emerging role of ISG15 in antiviral immunity."
Skaug B. , Chen Z.J.
Cell143:187-190(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: REVIEW
14.

"ISG15 modulates development of the erythroid lineage."
Maragno A.L. , Pironin M. , Alcalde H. , Cong X. , Knobeloch K.P. , Tangy F. , Zhang D.E. , Ghysdael J. , Quang C.T.
PLoS ONE6:E26068-E26068(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN STAT5A; MAPK1; PLC-GAMMA AND GLOBIN ISGYLATION
15.

"ISG15 is critical in the control of Chikungunya virus infection independent of UbE1L mediated conjugation."
Werneke S.W. , Schilte C. , Rohatgi A. , Monte K.J. , Michault A. , Arenzana-Seisdedos F. , Vanlandingham D.L. , Higgs S. , Fontanet A. , Albert M.L. , Lenschow D.J.
PLoS Pathog.7:E1002322-E1002322(2011) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
16.

"Mycobacterial disease and impaired IFN-gamma immunity in humans with inherited ISG15 deficiency."
Bogunovic D. , Byun M. , Durfee L.A. , Abhyankar A. , Sanal O. , Mansouri D. , Salem S. , Radovanovic I. , Grant A.V. , Adimi P. , Mansouri N. , Okada S. , Bryant V.L. , Kong X.F. , Kreins A. , Velez M.M. , Boisson B. , Khalilzadeh S. , more...
Science337:1684-1688(2012) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION

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